Accueil
 
Home > Actualite page d'accueil > BME Seminars - Emmanuel Beaurepaire

BME Seminars - Emmanuel Beaurepaire


Flyer_Emmanuel_Beaurepaire

On Friday the 11th of April 2014, the BME seminars "Open Your Mind" will host a conference of Emmanuel Beaurepaire from the Lab for optics and biosciences (Ecole Polytechnique - CNRS - Inserm, Palaiseau). His conference is entitled “Multicolor and multimodal nonlinear imaging of developing tissue”

 

The conference will take place on Friday 11th of april at Centre Universitaire des Saint Pères, 45 rue des Saint Pères, 75006 Paris (Salle R229), from 13h30 to 15h30.

Abstract:

Modern issues in systems biology (neuroscience, development) require tissue-scale measurements of multiple cell parameters. Since its introduction in the early 90s, multiphoton (or nonlinear) fluorescence microscopy has proven invaluable for tissue studies with its ability to provide 3D sub-cellular resolution in thick/live samples. However multiparametric nonlinear imaging has until recently remained challenging.

We will first illustrate how the combination of fluorescence with harmonics contrasts (SHG, THG) provides complementary information for applications such as early embryo imaging [1]. We will then discuss advances in multicolor imaging. We report on a strategy that provides optimal and simultaneous two-photon excitation of three chromophores with distinct absorption spectra using two synchronized pulse trains [2]. We report on volume and live multicolor imaging of 'Brainbow'-labeled tissues using this approach [2,3], opening the path to large-scale cell and lineage tracking. Finally, we will discuss the potential of additional advances such as wavefront control [4] and light-sheet excitation [5] to increase the imaging depth and speed of tissue imaging.    

 

References:

[1] Olivier et al, Science 329 (2010) p.967.

[2] Mahou et al, Nat Methods 9 (2012) p.815.

[3] Loulier et al, Neuron 81 (2014) p.505.

[4] Zeng et al, Biomed Opt Express 3 (2012) p.1898.

[5] Truong, Supatto et al, Nat Methods 8 (2011) p.757.

 


Arts et Métiers ParisTech
ESPCI
Mines
Telecom
ParisTech
Université Paris Descartes
Master BME Paris